PMC

Efficiency analysis of the surface decontamination in each location at four sampling times. The C _t values were used as an index and showed that DNA contamination decreased each day over the 2 week period. C _t values are inversely proportional to the concentration of the target DNA, and the greater the C _t value is, the lower the DNA concentration is. A C _t value of 30 indicates non‐amplification of the target DNA. (A) Surveillance of surface decontamination during the reagent preparation (first room). (B) Surveillance of surface decontamination in the gene amplification (third room). (C) Surveillance of surface decontamination in the specimen handling room (second room). (D) Surveillance of surface decontamination during product analysis (fourth room). (E) Surveillance of surface decontamination in the lobby ward (Buffer ward)

Impact of neuropil decontamination on neuronal responses measured with two-photon calcium imaging, in V1 layer 2/3 of awake behaving mice. Neurons were labelled with GCaMP6f. The effect of locomotion was quantified for each neuron by the locomotion modulation index (LMI). (A) An example field of view with somatic ROIs coloured by the LMI value obtained either before neuropil decontamination (‘Measured’) or after decontamination. The ROIs for the measured, subtraction, and FISSA methods are identical and were defined by hand. For the cNMF method the ROIs are presented as detected by the algorithm. LMI values were calculated for periods of visual stimulation with oriented gratings (10 to 20 trials per field of view, 60 s/trial). (B) The distributions of LMI values across all layer 2/3 cells in eight mice, before (‘Measured’) and after each decontamination method. (C) The distributions of median LMI values for the eight mice, before and after decontamination. *p < 0.05; n.s.: not significant (p > 0.05); Wilcoxon signed-rank test, n = 8 mice, FISSA vs measured: p = 0.0117, FISSA vs subtraction p = 0.0117, FISSA vs cNMF p = 0.0687, subtraction vs cNMF p = 0.0687.

Tukey’s box plot of endpoint fecal and cecal Richness (number of distinct OTUs) (A and B), Shannon Diversity Index (C and D), and Simpson diversity index (E and F). Bottom axis represents combination of bedding and water decontamination method: autoclaving with reverse osmosis (RO), autoclaving with hydrochloric acid (HCl), autoclaving with sulfuric acid (H2SO4), and autoclaving alone (Autoclaved). Asterisks represent significant main effect of bedding in two-way ANOVA (). Like lower case letters indicate significantly different (p<0.05) comparisons between groups as determined via two-way ANOVA followed by Tukey’s post hoc test. For example, the Corn cob/HCl and Paperchip/HCl groups are labeled with an “a” indicating that a significant difference was found between these two groups.